Composite

Part:BBa_K3456000

Designed by: Lina Schmidt   Group: iGEM20_Aachen   (2020-09-25)


I1-60GFPHis - ATPase Inhibitor with GFP and His-Tag

The part consists of an inhibitor sequence (I1-60) that can bind to ATPase. It inhibits the hydrolytic function of the ATPase but not the ATP synthesis. The inhibitor sequence originates from a bovine inhibitor protein IF1. Only the first 60 amino acid residues of this protein are needed for this purpose. The inhibitor sequence is fused with a 17x helix to a eGFP and a His-Tag. The whole part can be used to purify a ATPase with a affinity purification via the His-Tag.

The construct is controlled by a Lac-Operon including LacI, LacO and a T7 promoter and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None